Atkinson NJ et al. 1988
- Authors: Atkinson NJ, Ford-LLoyd BV, Newbury JH
- Title: Regeneration of plants from Antirrhinum majus L. callus.
- Location: Plant Cell Tissue and Organ Culture 17:59-70 (1988).
- Abstract: Somaclone production in Antirrhinum majus plants
by regeneration of plants from callus cultures has been achieved using
three types of explant tissue. Regeneration from mature stem
internode-derived callus was extremely poor. Callus derived from seedling
shoot tips could be induced to from new shoots in six of seven cultivars
tested. Regeneration was achieved in all seven cutivars when callus was
produced from seqments of hypocotyl and was most effective using
agar-solidified medium containing 0.25 mg /l naphthoxyacetic acid + 10%
coconut milk. In this case, five of the cultivars produced shoots
directly, one produced leaves from the petioles of which new shoots
emerged, and one regenerated plants chiefly through the production of
embryoids.
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