Isolation of aureusin and apigenin
This method shows the isolation of the yellow flower
pigments aureusin and apigenin 7-glucuronide from snapdragons.
The method has been published in Asen S.
et al. 1972.
- Take petals of yellow snapdragons (for instance cultivar "Yellow Rocket")
- Extract with Methanol.
- Reduce volume at 40 degrees C und reduced pressure.
- Take up residue in citrate-phosphate buffer (pH 3.0)
- Filter with celite
- Absorb in 25 X 400 mm column of purified insoluble polyvinylpyrrolidone
(polyclar AT) with fines removed.
- Wash column with 200 ml Water.
- Elute pigments with Methanol containing 1ml of 2 N HCl per liter.
- Apigenin 7-glucoronide elutes after 24 hours, but is esterified.
- Aureusin elutes after 3 days.
- Hydrolize Apigenin methyl ester by dissolving in 0.5 N NaOH and
place on a stean math thor 30 min. Cool, acidify and crystallize from
aqueous solution.
- Purify Aureusin using a 35 X 450 Cellulose column. Elute with
EtOAc:2-PrOH:HCOOH:Water (10:5:2:3). Crystallize from aqueous alcohol.
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